PFASs are a group of synthetic organofluorine chemical compounds used in the
production of fluoropolymer coatings and products such as furniture, adhesives,
personal care products, food packaging, mobile phone screens, etc. Many PFASs
pose health and environmental concerns because they are classified as persistent
organic pollutants. The long-chain PFASs are due to higher bioaccumulative potential
frequently detected in living organisms. This application shows a robust method of
extraction and determination of the PFAS compounds in fish biota samples, which
can be a relevant exposure pathway for humans.
MSPE with 15 mg of C18 stationary phase and nylon frits was used to analyze 14 PFAS
compounds in fish tissue samples. The MSPE sample preparation approach was
compared with the reference extraction method without SPE based on Grabicova
et al. 2018 [1]. Both sets of samples were fortified to 1 ng/g of PFAS. The MSPE
method enhances the response of most of the analytes between 80 –130 % (Fig. 1).
Although sample preparation is more demanding than the reference method, the
MSPE offers relevant signal improvement.
This application was developed in cooperation with the Faculty of
Fisheries and Protection of Waters, Research Institute of Fish Culture and
Hydrobiology, Laboratory of Environmental Chemistry and Biochemistry.
